How to isolate vascular smooth muscle cell?
Posted 18 February 2010 - 02:02 AM
Posted 18 February 2010 - 08:40 AM
From a primary culture of vascular smooth muscle cell, I have obtained VSMCs and other cellular types. How can I isolate only VSMCs and discard the other one? I know that the major type is smooth muscle cells because I done immunocytochemistry (alpha-actin).
Live-cell FACS or magnetic bead sorting should allow you to purify your population if you have a specific marker for your cells of interest.
then heaven will be yours, before you meet your end