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confocal lymphoblast studies


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#1 shldbcrzy

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Posted 17 February 2010 - 08:58 AM

I was trying to stain lymphoblasts for confocal studies, but the problem is that i do not know how to immobilize them on the slide. Does any one work on such free floating cells

#2 SuMi

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Posted 18 February 2010 - 10:09 AM

I use Polysine slides and allow the cells to adhere for 30 minutes before staining. You could also coat the slides or coverslips with poly-L-lysine

#3 shldbcrzy

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Posted 18 February 2010 - 03:14 PM

thank u. I will try that




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