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confocal lymphoblast studies
2 replies to this topic
Posted 17 February 2010 - 08:58 AM
I was trying to stain lymphoblasts for confocal studies, but the problem is that i do not know how to immobilize them on the slide. Does any one work on such free floating cells
Posted 18 February 2010 - 10:09 AM
I use Polysine slides and allow the cells to adhere for 30 minutes before staining. You could also coat the slides or coverslips with poly-L-lysine