Caco-2 passage number for MTT?
Posted 16 February 2010 - 08:14 PM
I'm totally new with cell assay and not sure how "old" my caco-2 cells could be for MTT assay. Could someone please help me on this, or maybe show me where I could find the answer?
Thanks a lot
Posted 17 February 2010 - 03:11 PM
Posted 18 February 2010 - 12:40 AM
thanks for your reply but could you elaborate more please? i'm a bit confused here...
i thought that selection occurs every time we subculture and cell functions will change significantly at higher passage number, hence cell viability of low- and high- passage is no longer comparable? please enlighten me >_<
Posted 21 February 2010 - 03:51 PM
Posted 22 February 2010 - 07:52 PM
Oh i get it now! Thanks a lot Bob