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low serum media and FCS

Started by deva.kumari, Feb 16 2010 05:14 AM

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2 replies to this topic

#1 deva.kumari

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Posted 16 February 2010 - 05:14 AM

Hi,
Is it necessary to use a collagen coated plate for the propagation of mammalian cells grown in low serum media????

thank you

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#2 rhombus

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Posted 16 February 2010 - 05:32 AM

deva.kumari, on Feb 16 2010, 01:14 PM, said:

Hi,
Is it necessary to use a collagen coated plate for the propagation of mammalian cells grown in low serum media????

thank you

Only if the specific cells you are trying to grow have problems with adherence.

Rhombus

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#3 deva.kumari

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Posted 16 February 2010 - 06:15 AM

thank you for your response...
i am trying to culture primary renal cells..the media contains 0.5% FBS..i have not handled renal cells before...
normally primaries have a problem in revival after freezing...so do i go on with normal flasks????
thank you