Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

0

low serum media and FCS

Started by deva.kumari, Feb 16 2010 05:14 AM

Please log in to reply

2 replies to this topic

#1 deva.kumari

member

Active Members
6 posts

0
Neutral

Posted 16 February 2010 - 05:14 AM

Hi,
Is it necessary to use a collagen coated plate for the propagation of mammalian cells grown in low serum media????

thank you

Back to top

#2 rhombus

Rhombus/Uncle Rhombus

Active Members
203 posts

13
Good

Posted 16 February 2010 - 05:32 AM

deva.kumari, on Feb 16 2010, 01:14 PM, said:

Hi,
Is it necessary to use a collagen coated plate for the propagation of mammalian cells grown in low serum media????

thank you

Only if the specific cells you are trying to grow have problems with adherence.

Rhombus

Back to top

#3 deva.kumari

member

Active Members
6 posts

0
Neutral

Posted 16 February 2010 - 06:15 AM

thank you for your response...
i am trying to culture primary renal cells..the media contains 0.5% FBS..i have not handled renal cells before...
normally primaries have a problem in revival after freezing...so do i go on with normal flasks????
thank you