hi! It's my first time to handle cell culture and I don't really have much background about it. I just wanna ask if how can i maintain a healthy culture of MCF-7 cells? How do I know if my cells are still healthy or not? dead or alive? can you show me pictures of healthy, unhealthy, and dead cells? because my MCF-7 cultures are mostly round and small, like they have shrunk a bit, and not like what i can see in the ATCC photo, which is elongated. many also have lots of dots in them. moreover, my cells are often very dense and there are so many clumping. I regularly washing them or feed them every two days when they are not yet confluent to remove floating cells and to lessen the clumping, yet they are still clumping so much I cannot make a clean monolayer of cells. please help me.
PS. attached is a photo of my MCF-7 cells.. sorry if its quite unclear.. i was using my own camera...
Edited by msfive, 15 February 2010 - 09:15 AM.