cell treatment rules
Posted 12 February 2010 - 10:29 AM
I'd like to analyze the apoptotic effect of a peptide molecule on cultured cells. I usually use 12 hour serum-starved cells and I apply the compound dissolved in serum-free medium up to 96 hours. I'm afraid that using a serum-free medium during a long treatment can affect health of cells even in the absence of the compound.
Do you think I'm right when using serum-free medium during treatments? Can you suggest some general rules to conduct treatments in cell cultures? Is absence of serum essential during tests? What compounds contained in standard medium must be avoided in treatment media?
Thank you very much for your replies!
Posted 14 February 2010 - 03:35 PM
Posted 15 February 2010 - 01:48 AM
Thanks a lot!
Posted 15 February 2010 - 03:47 PM
If you are using a commonly used cell line such as 293, A549, MCF-7, i would try and see if the cells function normally with reduced serum at a range of concentrations and then use the minimum possible.
Posted 16 February 2010 - 08:31 AM
Thanks a lot for your suggestion!