I am trying to culture macrophages from whole blood. What I am looking for is a way to assess the purity of my macrophage population adhered to the culture dish.
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#2
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Posted 27 June 2002 - 05:56 PM
Use Mac-1 and GR-1(granulocytes) antibodies and do FACS analysis or something like that.
#3
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Posted 24 November 2003 - 03:43 AM
Alternatively, have you ever tried to generate macrophages from monocytes in the presence of GM-CSF ?
#4
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Posted 19 December 2003 - 02:45 PM
Can also try diI labeled acetylated LDL to test their phagocytic activity.
#5
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Posted 29 November 2004 - 07:24 AM
Time of adheration and washing of adhered monocytes are very important. Standardized it.
