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Macrophage culture


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4 replies to this topic

#1 Sinead

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Posted 08 June 2002 - 05:41 AM

I am trying to culture macrophages from whole blood. What I am looking for is a way to assess the purity of my macrophage population adhered to the culture dish.

#2 RichardColes

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Posted 27 June 2002 - 05:56 PM

Use Mac-1 and GR-1(granulocytes) antibodies and do FACS analysis or something like that.

#3 febstein

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Posted 24 November 2003 - 03:43 AM

Alternatively, have you ever tried to generate macrophages from monocytes in the presence of GM-CSF ?

#4 Victor

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Posted 19 December 2003 - 02:45 PM

Can also try diI labeled acetylated LDL to test their phagocytic activity.

#5 rakeshbhu

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Posted 29 November 2004 - 07:24 AM

Time of adheration and washing of adhered monocytes are very important. Standardized it.




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