Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

contaminated esi mass spec samples


  • Please log in to reply
No replies to this topic

#1 rachelg

rachelg

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 11 February 2010 - 04:23 PM

hi,

i am trying to analyse culture media by esi ms and keep getting a contamination peak (353.12 and 705.23 in particular). According to the ms people it is not a peptide or lipid because its spectrum is too simple. I have been looking at common contaminant tables (plasticizers etc) and the m/z associated with those contaminants, so far i have not seen my contaminant.

i have bought new media stocks and tested them only to find the same contaminant. The MilliQ water that the media is made up in does not have the contamination. we use a previously tested amount of BSA so that it doesn't block the signal of less abundant proteins and a previous researcher was getting this to work, but i don't know what has changed apart from me.

The attached spectra are (1) media with BSA, (2) milliQ and (3) media without BSA.


Thanks in advance for any suggestions

rachel

Attached Files


Edited by rachelg, 11 February 2010 - 04:24 PM.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.