Transfection before plating?
Posted 11 February 2010 - 09:16 AM
Does anybody know a protocol how to transfect dissociated neurons before plating? I don't mean electroporation but some other technique like e.g. lipofectamine.
Somehow in the depths of my brain i remember having seen a protocol how to do this but if it really exists i can not find it any more.
The point is that i'd like to transfect neurons plated on glial feeder layers but the Ca-phosphate protocol i use gives me a tremendous background of nicely transfected glia cells. So if i could find a way to transfect the cells before plating, this would be great.
Any comments are appreciated!
Posted 11 February 2010 - 03:25 PM
Edited by bob1, 11 February 2010 - 03:27 PM.
Posted 12 February 2010 - 02:18 AM
Basically this is my plan. I made the experience that i get nice expression already after 25 min of incubation with lipofectamin/DNA. So i hope i'll have enough cells that survive this procedure and get transfected.
I will post my results here.