I have serious problem with gel purification.
I am trying to remove the insert-beta actin- from my vector-RFP ruby.However, for last three weeks such a problem emerged that even I do double purification there are some inserts in my DNA vector.
As a result of test digestions, we understood that restriction digestions work well.It is suprising that the QIAGen Gel purification kit was working well one month ago.We tried same kit last week with another unrelated vector, the same problem we have seen.I haven't changed anything from protocol and anything from solutions.
What do you think?
You can find the process in the attachment.I appreciate all kind of help.
Emergent replies please...