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Primers for pre-mRNA


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#1 Spliffeh

Spliffeh

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Posted 11 February 2010 - 04:31 AM

Hy everyone,
does anyone here have any experience in developing primers for pre-mRNA?
The RNA is acquired with a total RNA extraction protocol and is than reverse transcriptase'd to cDNA using random hexamer primers.
The problem here of course is that there might be gDNA contamination...
But first things first, how do you best start making primers for pre-mRNA or is there an alternative way to do this?

Cheers




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