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cell lysis for metabolite quantification

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#1 themoon



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Posted 10 February 2010 - 07:24 PM

I have a bunch of monocyte cells and I want to lyse them so that I can determine the quantity of specific metabolites using GC/MS. I have them in 100ul PBS. I was wondering what the best protocols are either sonicating or using lysis buffer..?

Thanks very much!

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