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[woeiyih]

I had spent alot of time on screening the uptream o my gene by colony hybridization.
However, the transform efficiency is very low..
My DNA fragments is about 3.8kb....
How can I clone the upstream or downstraem of my gene??
Please help me.

[milanoj]

You will need to perform a process called inverse PCR.

        Partial Inverse PCR: A Technique for Cloning Flanking Sequences
                             
        Pang, K. M. Knecht, D.A.
        Published Date: 06/20/1997, Benchmarks, BioTechniques 22:1046-1048