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[trinekal]

Anybody out there who can help me?  Looks like my "total binding" is too high. I dont know how to solve this, any suggestions?

Duplicate
Tube cpm readings
TC 15975 20104 18040
NSB 152 141 147
Bo = (TB- NSB) = 14176

Standard Curve cpm Average Av - NSB µU/ml B/Bo FIT

TB 14553 14091 14322 14176 0,001 1,000 1,000

H 13397 14417 13907 13761 2,000 0,971 0,948
G 10103 10951 10527 10381 5,000 0,732 0,860
F 8672 11768 10220 10074 10,000 0,711 0,733
E 7898 7071 7485 7338 20,000 0,518 0,560
D 6797 5260 6029 5882 50,000 0,415 0,340
C 3753 3851 3802 3656 100,000 0,258 0,231
B 3195 2203 2699 2553 200,000 0,180 0,172

Edited by trinekal, 10 February 2010 - 01:15 AM.