Has anyone had problems with tranformation efficiency when purchasing these cells? There is a single supplier and I have had nothing but problems with these cells. I am about to make my own cells, but do not have time to do that on a regular basis. When I call the company supplying the cells, they claim that they do not have problems with efficiency. They are not a commonly used strain and the replacement lot is the same as the bad lot they sent in the first place. They do not know when they will have a new lot available. Yes, I have transformed using pUC19 to test efficienty and it is horrible- with no colonies at all the last time I tested. I would just like to validate with someone else that I have spent megabucks for useless cells, and wasted a lot of time. GT115 has its own set of problems. Unfortunately, with the R6K origin, these cells are the only option. I have been using these cells for the last 10+ yrs- it is not a matter of technique etc. I have tested a ligation mix that previously gave a ton of colonies with a previous lot of cells and got 1 colony. This is very frustrating.
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