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MTT assay problem...!!! :(


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#1 olga_m

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Posted 09 February 2010 - 04:09 AM

I tried MTT assay with cancer cells (A549 & SHSY-5Y), and tried the isopropanol/HCl to dissolve the formazan, but the cellular debris still remains in the well and i think this causes interference in OD readings. :)
How can I get rid of that?

Thank you soooo much!!! ;)

#2 jennifer.i.hr

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Posted 11 February 2010 - 10:21 AM

I currently use MTT for my cytotoxicity assays and I use DMSO for the readings. Have you tried this?




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