How can I get rid of that?
Thank you soooo much!!!
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MTT assay problem...!!! :(
Started by olga_m, Feb 09 2010 04:09 AM
1 reply to this topic
#1
olga_m
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Posted 09 February 2010 - 04:09 AM
I tried MTT assay with cancer cells (A549 & SHSY-5Y), and tried the isopropanol/HCl to dissolve the formazan, but the cellular debris still remains in the well and i think this causes interference in OD readings.
How can I get rid of that?
Thank you soooo much!!!
How can I get rid of that?
Thank you soooo much!!!
#2
jennifer.i.hr
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Posted 11 February 2010 - 10:21 AM
I currently use MTT for my cytotoxicity assays and I use DMSO for the readings. Have you tried this?
