I tried MTT assay with cancer cells (A549 & SHSY-5Y), and tried the isopropanol/HCl to dissolve the formazan, but the cellular debris still remains in the well and i think this causes interference in OD readings.
How can I get rid of that?
Thank you soooo much!!!
Submit your paper to J Biol Methods today!
MTT assay problem...!!! :(
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