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Hepes-KOH buffer


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#1 szang

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Posted 08 February 2010 - 06:35 PM

hi everyone,

I want to make Hepes-KOH buffer, but protocols always suggest me to filter the buffer not to autoclave it. Dose anyone know why?

thanks








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#2 Inmost sun

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Posted 13 April 2010 - 09:09 AM

hi everyone,

I want to make Hepes-KOH buffer, but protocols always suggest me to filter the buffer not to autoclave it. Dose anyone know why?

thanks


heat (and even long-time storage) modifies HEPES; old HEPES gets light browny; I think of oxidations...





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#3 NHI

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Posted 13 July 2010 - 12:31 AM

hi fren....

from my own experience...

better for u to sterile filter all buffers rather than autoclave due to changes of physical properties pH and conductivity sometimes and more serious for chemicals properties of the combine chemicals such as oxidation and so on...




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