Problem with protein samples
Posted 08 February 2010 - 03:33 PM
Posted 09 February 2010 - 12:01 AM
Did you do a solvent/buffer control in the BCA to control for background BCA turnover?
Posted 09 February 2010 - 12:28 PM
how are you detecting the protein in the gel? coomassie? silver? immunostain? something else?
your protein load may be too little to detect by the method you are using. if you are using silver, i have seen proteins that required a second round of silver staining to become visible.
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