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Analyzing qPCR results


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#1 serundipity2009

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Posted 08 February 2010 - 09:11 AM

Hi all,
I am doing qPCR to look at whether this gene is upregulated or downregulated in WT and Mut mice. How do I calculate the relative gene expression after i calculated the Ct value for the mutant and wild type?

THanks !!!

#2 susanna

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Posted 10 February 2010 - 08:15 AM

Hi all,
I am doing qPCR to look at whether this gene is upregulated or downregulated in WT and Mut mice. How do I calculate the relative gene expression after i calculated the Ct value for the mutant and wild type?

THanks !!!



using analyse type: relative quantification in the lightcycler menu




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