1 reply to this topic

[serundipity2009]

Hi all,
I am doing qPCR to look at whether this gene is upregulated or downregulated in WT and Mut mice. How do I calculate the relative gene expression after i calculated the Ct value for the mutant and wild type?

THanks !!!

[susanna]

serundipity2009, on Feb 8 2010, 10:11 AM, said:

Hi all,
I am doing qPCR to look at whether this gene is upregulated or downregulated in WT and Mut mice. How do I calculate the relative gene expression after i calculated the Ct value for the mutant and wild type?

THanks !!!

using analyse type: relative quantification in the lightcycler menu