Hi
I am going to clone a 1.8 kb gene is it safe to amplify it with long pcr mix (fermentas) or high fidelity enzyme mix (roche) or I should aplify it only with pfu?
regards
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Posted 07 February 2010 - 04:46 AM
sara.r, on Feb 7 2010, 03:27 PM, said:
Hi
I am going to clone a 1.8 kb gene is it safe to amplify it with long pcr mix (fermentas) or high fidelity enzyme mix (roche) or I should aplify it only with pfu?
regards
I am going to clone a 1.8 kb gene is it safe to amplify it with long pcr mix (fermentas) or high fidelity enzyme mix (roche) or I should aplify it only with pfu?
regards
Hi,
pfu should be quite ok for your purpose.
