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Cycloheximide


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#1 romero2010

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Posted 06 February 2010 - 11:53 AM

Hello everyone!!!

Could you please tell me some controls for the CHX-assay?

I am working with a seems-to-be-stable protein that its levels don't go down even after using 100 microgr/ml of CHX for 24 hrs. Is there a/some controls to show that the assay works (meaning a short lived protein)?

Thank you very much in advance!!!

#2 bob1

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Posted 07 February 2010 - 03:54 PM

tp53 has a half life of approx 0.5 hours and is induced between 8 and 12 hours post UV treatment.




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