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dam methylated DNA in dam - straights


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#1 Stuck with Ligation

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Posted 05 February 2010 - 12:08 PM

I have a vector that is in a glycerol stock of a dam+ strain. This methylates my XbaI site. If I transform this purified plasmid from a dam+ strain into a dam- strain will the methylation be removed?

#2 HomeBrew

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Posted 05 February 2010 - 12:11 PM

Yes -- as the plasmid is replicated in the dam- strain, the newly-synthesized DNA will not be dam methylated (that's not to say the DNA will be entirely unmethylated, just that dam methylation will be absent).




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