I have a vector that is in a glycerol stock of a dam+ strain. This methylates my XbaI site. If I transform this purified plasmid from a dam+ strain into a dam- strain will the methylation be removed?
0
1 reply to this topic
#1
Stuck with Ligation
-
- Active Members
-
- 33 posts
Enthusiast
0
Neutral
Neutral
Posted 05 February 2010 - 12:08 PM
#2
HomeBrew
-
- Global Moderators
-
- 927 posts
Veteran
14
Good
Good
Posted 05 February 2010 - 12:11 PM
Yes -- as the plasmid is replicated in the dam- strain, the newly-synthesized DNA will not be dam methylated (that's not to say the DNA will be entirely unmethylated, just that dam methylation will be absent).
