Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

dam methylated DNA in dam - straights

  • Please log in to reply
1 reply to this topic

#1 Stuck with Ligation

Stuck with Ligation


  • Active Members
  • PipPipPipPipPip
  • 33 posts

Posted 05 February 2010 - 12:08 PM

I have a vector that is in a glycerol stock of a dam+ strain. This methylates my XbaI site. If I transform this purified plasmid from a dam+ strain into a dam- strain will the methylation be removed?

#2 HomeBrew



  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 930 posts

Posted 05 February 2010 - 12:11 PM

Yes -- as the plasmid is replicated in the dam- strain, the newly-synthesized DNA will not be dam methylated (that's not to say the DNA will be entirely unmethylated, just that dam methylation will be absent).

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.