have problems with electroporation transformation of PCP20 into BL21 de3
Posted 05 February 2010 - 09:03 AM
I used electroporation transformation to introduce pCP20 into BL21 DE3 and get no colony on the selective meium. but when I did the same procedure with K strains like K12, it works well. I don't know why. Does anybody have a great idea to make the transformation successful?
Posted 10 February 2010 - 08:58 AM
It really helps
Posted 10 February 2010 - 09:07 AM
by the way, you incubate ur cells at what temperature?