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have problems with electroporation transformation of PCP20 into BL21 de3


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#1 clonver

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Posted 05 February 2010 - 09:03 AM

Hi there,
I used electroporation transformation to introduce pCP20 into BL21 DE3 and get no colony on the selective meium. but when I did the same procedure with K strains like K12, it works well. I don't know why. Does anybody have a great idea to make the transformation successful?


Thanks,

clonver

#2 arien

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Posted 10 February 2010 - 08:58 AM

did u talk with the company where u bought the cells ?
It really helps
Arien

#3 badguy

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Posted 10 February 2010 - 09:07 AM

play around with the voltage and pulse length, or even the number of pulses.

by the way, you incubate ur cells at what temperature?




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