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Protein localization


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#1 molecule

molecule

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Posted 05 February 2010 - 12:46 AM

Hi, I;m new to working with proteins and westerns etc; I have a mutant which has a N terminus truncation of a helicase protein and I need to study the localization of this protein on the replication fork. Can some one give me some hints as to how I should et about starting on this. I think I can try to do an IP with it via an antibody; but how to do that or any other suggestions.?
thnaks

#2 bob1

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Posted 07 February 2010 - 03:56 PM

Cellular fractionation - e.g. nuclear fractionation, followed by western. You could also do it by immunofluorescence. Both methods require you to have antibodies against the mutant and normal proteins.




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