Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

using cell lysate to run native gel?

  • Please log in to reply
1 reply to this topic

#1 happy potter

happy potter


  • Active Members
  • Pip
  • 5 posts

Posted 04 February 2010 - 09:02 PM

If the protein I am interested in has a pI of 9.5 , can I use acidic native gel to separate it from other cellular protein? I think I will use western to detect it following the native gel. I know there is acetate acidic native gel, so shall I directly add the sample loading buffer (~pH4.3, containing acetate) to the cell lysate and run it?
Thanks alot for answering.

#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,259 posts

Posted 05 February 2010 - 07:52 AM

are you using a stacking gel?

if so, then you will want to adjust your sample to near the pH of the stacking gel, rather than that of the running gel.
talent does what it can
genius does what it must
i do what i get paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.