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using cell lysate to run native gel?


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#1 happy potter

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Posted 04 February 2010 - 09:02 PM

If the protein I am interested in has a pI of 9.5 , can I use acidic native gel to separate it from other cellular protein? I think I will use western to detect it following the native gel. I know there is acetate acidic native gel, so shall I directly add the sample loading buffer (~pH4.3, containing acetate) to the cell lysate and run it?
Thanks alot for answering.

#2 mdfenko

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Posted 05 February 2010 - 07:52 AM

are you using a stacking gel?

if so, then you will want to adjust your sample to near the pH of the stacking gel, rather than that of the running gel.
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