If the protein I am interested in has a pI of 9.5 , can I use acidic native gel to separate it from other cellular protein? I think I will use western to detect it following the native gel. I know there is acetate acidic native gel, so shall I directly add the sample loading buffer (~pH4.3, containing acetate) to the cell lysate and run it?
Thanks alot for answering.
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mdfenko
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Excellent
Excellent
Posted 05 February 2010 - 07:52 AM
are you using a stacking gel?
if so, then you will want to adjust your sample to near the pH of the stacking gel, rather than that of the running gel.
if so, then you will want to adjust your sample to near the pH of the stacking gel, rather than that of the running gel.
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