2 replies to this topic

[ragno]

Hello,

I'am doing to do a southern blotting and i have forgotten to crosslink the DNA on membrane before the prehybridisation step.I would like to know if DNA is always on membrane without crosslinking.Thanksmichel

[rhodadg]

There will be some DNA but don't expect distinct bands when you probe

[jbak]

It depends on the transfer method and the type of membrane. If you did alkali blotting onto N plus membrane it is not necessary to crosslink your DNA because it is covalently linked.