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2 replies to this topic

#1 ragno

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Posted 26 October 2001 - 09:00 PM

Hello,

I'am doing to do a southern blotting and i have forgotten to crosslink the DNA on membrane before the prehybridisation step.I would like to know if DNA is always on membrane without crosslinking.Thanksmichel


#2 rhodadg

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Posted 16 June 2002 - 01:19 PM

There will be some DNA but don't expect distinct bands when you probe

#3 jbak

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Posted 25 June 2002 - 03:42 AM

It depends on the transfer method and the type of membrane. If you did alkali blotting onto N plus membrane it is not necessary to crosslink your DNA because it is covalently linked.




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