Posted 04 February 2010 - 06:24 AM
I've been trying for some time to complete SSCP, but still haven't succeeded til the very end. The problem is that everything goes almost as it should be, but in the end I never get the zones to show up, the gel itself is in the necessery colour, but even gene ruler can't be seen. Has anyone tried to use this method and also had some problems? What problems could there possibly be? I've gone through the protocol over and over again, but still don't know what could be the reason for this.
Posted 04 February 2010 - 06:58 AM
Posted 05 February 2010 - 05:01 AM