1 reply to this topic

[katenkak]

Dear all,
I am going to measure flluorescense of AMCA, but I have only black plates with transparent bottom. Can I cover the bottom (with adhesive sheet) and still measure fuorescense or I can use ONLY whole black plates with black bottom?
Thanks for answering!

[phage434]

You could cover the bottom, but you have to be careful about what material you choose.  Many materials are fluorescent, and you have to avoid those.  A blank would reveal excessive fluorescence in the blocking material.  It's possible that simply using the plates as-is would be the best solution.  I think the inside of your plate reader is likely to be quite dark.  There may be extra well-well bleeding of light, but I would guess that to be minimal.