I am going to measure flluorescense of AMCA, but I have only black plates with transparent bottom. Can I cover the bottom (with adhesive sheet) and still measure fuorescense or I can use ONLY whole black plates with black bottom?
Thanks for answering!
1 reply to this topic
Posted 04 February 2010 - 05:05 AM
You could cover the bottom, but you have to be careful about what material you choose. Many materials are fluorescent, and you have to avoid those. A blank would reveal excessive fluorescence in the blocking material. It's possible that simply using the plates as-is would be the best solution. I think the inside of your plate reader is likely to be quite dark. There may be extra well-well bleeding of light, but I would guess that to be minimal.