12345678, on Feb 3 2010, 02:13 PM, said:
Hi! I have a cell line from which I did a membrane extract preparation, and then an immunoprecipitation. Ran the gel, sent it to mass spec and they can only identify BSA. How can I get rid of BSA? Should I wash the cells, use another kind of serum or just use those BSA removal kits?
try washing before trying the removal kits.
( they always state that removal of bovine albumin is not optimal... how not optimal??)
most removal kits will also remove other proteins along with bsa (they use a dye which binds bsa preferentially but not exclusively). if your protein of interest is in low abundance then it may become undetectable if you use the kit.
beckman-coulter is marketing a kit which uses igy to bind bsa. it appears to be specific but it is probably expensive. i would only use it as a last resort.