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phenol red in media


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5 replies to this topic

#1 z0z

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Posted 02 February 2010 - 11:12 PM

can anybody tell me when to use phenol red free media.......is there any reason apart from toxicity

#2 jwickenden

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Posted 03 February 2010 - 04:16 AM

I use it with cells containing a ER fusion protein. Phenol red can mimic tamoxifen and activate the protein.
don't know of any other reason

#3 KevinK

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Posted 03 February 2010 - 06:57 AM

Phenol Red, being a colored compound, absorbs light. Phenol Red-free media may absorbe light from many assays such as absorbance and luminescence and may autofluorece with some excitation lights in addion to absorbing emission.

Kevin
Promega Corporation
Madison, WI

#4 bob1

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Posted 03 February 2010 - 04:08 PM

Phenol red can mimic tamoxifen and activate the protein.

Phenol red is a weak estrogen analogue - it will cause the estrogen receptor to internalize. Tamoxifen is an antagonist - it blocks but does not activate the receptor.

#5 z0z

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Posted 03 February 2010 - 09:45 PM

so that means i have to use phenol red free media when i am working on cell lines that respond to estrogen

#6 bob1

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Posted 04 February 2010 - 03:50 PM

correct




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