2 replies to this topic

[sdar]

i want to kow that how many basepair must be flanking a restriction site in order to restrict it with type 2 restriction enzymes and how many basepairs should be flanking a gene in order to clone it in an expression vector. does number of flanking basepairs matter

[bob1]

Have a look at the attached table, it should help.

Attached Files

•  extensions.pdf   277.52K   61 downloads

[KAUSHIK THAKKAR]

Good one..
Or you can check this, if you think above is not authentic..
http://www.neb.com/n...nucleotides.asp