Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

miRNA isolation


  • Please log in to reply
3 replies to this topic

#1 labgrl76

labgrl76

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 01 February 2010 - 01:48 PM

Can anyone please tell me if microRNAs are isolated along with larger RNA molecules using the guanidinium isothiocyanate / caesium chloride centrifugation RNA extraction protocol?

I have previously extracted RNA from frozen cell line material using this method. I am now interested in microRNA and was wondering if my previously extracted RNA would also contain these small molecules.

#2 Fizban

Fizban

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 63 posts
0
Neutral

Posted 02 February 2010 - 12:45 AM

Can anyone please tell me if microRNAs are isolated along with larger RNA molecules using the guanidinium isothiocyanate / caesium chloride centrifugation RNA extraction protocol?

I have previously extracted RNA from frozen cell line material using this method. I am now interested in microRNA and was wondering if my previously extracted RNA would also contain these small molecules.


General semi-serious answer (but basically true):
if you see RNA there WILL be microRNAs as well. If you don't see RNA microRNAs will be there anyway!
It's just to say that if you do not use something that specifically eliminates short RNAs you will almost surely find miRNAs in your total RNA. sometimes you can detect small RNAs even in bad quality RNA samples.
just give it a try
Fiz :P

#3 Gaetanodiitaly

Gaetanodiitaly

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 19 February 2010 - 03:05 PM

SEE

MicroRNA isolation and stability in stored RNA samples.

Mraz M, Malinova K, Mayer J, Pospisilova S.

Biochem Biophys Res Commun. 2009 Dec 4;390(1):1-4. Epub 2009 Sep 19. Review.

#4 Biodate

Biodate

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 12 March 2010 - 08:23 AM

Not all RNA isolation kits will isolate Total RNA including miRNA. The only kit I use for this purpose is from Norgen Biotek. Otherwise you must use a phenol based method




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.