
Rate Zonal Centrifugation (density gradient)
#1
Posted 31 January 2010 - 02:51 PM
#2
Posted 01 February 2010 - 07:32 AM
Edited by mdfenko, 01 February 2010 - 07:32 AM.
genius does what it must
i do what i get paid to do
#3
Posted 02 February 2010 - 01:45 PM
the sample's unit density will be greater than the dense solution through which you are pushing it. it will, given enough time and centrifugal force, pellet.
TI don't understand how this is different than equilibrium-gradient centrifugation (aka isopycnography) , which is based on the same premise of using a gradient. Only when you centrifuge, everything separates based on density.
Edited by Ahrenhase, 02 February 2010 - 01:46 PM.
#4
Posted 03 February 2010 - 08:09 AM
yes. but if you let it continue the "bands" will continue to migrate towards the bottom of the tube, albeit slowly.TI don't understand how this is different than equilibrium-gradient centrifugation (aka isopycnography) , which is based on the same premise of using a gradient. Only when you centrifuge, everything separates based on density.
genius does what it must
i do what i get paid to do