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What does "seeding" mean?


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#1 prof. moriarty

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Posted 31 January 2010 - 01:23 PM

Beginner to cell culture - please help! What does "seeding" mean?

Edited by prof. moriarty, 31 January 2010 - 01:23 PM.


#2 Chakchel

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Posted 01 February 2010 - 01:21 AM

Seeding simply means to spread a defined amount (volume or cell number) of a cell suspension into a new flask or onto a plate etc.
When you work with adherent cell cultures you have to trypsinize them first to get a cell suspension.




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