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Re: oligo probe


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#1 anonymous

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Posted 04 April 2001 - 09:00 PM

Hi,does anyone have experience with crosslinking proteins in cells or cell extracts?

Thanx in advance


#2 anonymous

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Posted 06 April 2001 - 09:00 PM

I don't think you can do crosslinking for crude protein extract because there is lots lots of proteins being able to participate the crosslinking reaction. Pierece is a good company to make crosslinking reagents.

#3 anonymous

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Posted 05 May 2001 - 09:00 PM

Try precipitation with TCA and deoxycholate (5:1:1) of protein:72%TCA : 0.15% Deoxycholate).Dry different methods of protein estimation. I guess lowry's and Bicinchoninic acid methods are best in ur case.Good luckMomin




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