Why is the y-axis labeled 'relative gene expression'?
Posted 29 January 2010 - 05:53 PM
Posted 31 January 2010 - 02:46 PM
In a publication showing qRT-PCR data, they typically label the y-axis 'relative gene expression', why is that? Gene expression assumes that the gene is translated. Reverse Transcription qRT-PCR data shows the amount of polyadenylated transcript present but says nothing about the amount of which is translated into protein.
so i guess it's just a misnomer
Posted 31 January 2010 - 11:34 PM
Posted 01 February 2010 - 06:56 PM
Posted 02 February 2010 - 01:38 PM
Posted 02 February 2010 - 05:01 PM
According to wikipedia a gene is "a locatable region of genomic sequence, corresponding to a unit of inheritance, which is associated with regulatory regions, transcribed regions, and or other functional sequence regions . So perhaps gene does not imply protein? Doesn't really clear up the term "gene expression" though as a gene appears to refer to a "unit of inheritance", whatever that means.
But back to your point about a functional product, I don't know that we can say any mRNA, even if not translated into a protein, is not associated with some sort of functional product (what about all those introns spliced out?! Seems like a waste to me if it's not used for something!)...I'm of the persuasion that the cell does not run around making stuff for no reason.
But anyway, I digress....