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Ethidium bromide in formaldehyde gel?


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#1 JackWhisper

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Posted 28 June 2002 - 02:28 AM

Simple question: What is the main reason why ethidium bromide is not added to the formaldehyde gel but rather to the sample buffer in Northern hybridization protocol? Thanks.

#2 Keitaro

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Posted 28 June 2002 - 04:46 AM

Once the gel is stained with EtBr after running, it takes long time until the bachground becomes clear (I used to stain for 10 min, then soaked in water overnight).




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