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Simple question on LC and MS


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4 replies to this topic

#1 Ahrenhase

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Posted 29 January 2010 - 02:53 PM

I have to do a quick compare and contrast of protein identification techniques and I'm unsure of 2 necessary pieces of information due to lack of experience.

1)How long approximately does it take to prepare and run a liquid chromatography sample?

2)How long approximately does it take to prepare and run a mass spec sample (MALDI or ES)?

3)Are the components needed to run them expensive?

#2 Eve

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Posted 02 March 2010 - 01:50 PM

HPLC sample preparations can be short (run directly) for some methods like size exclusion, or longer (perform extraction or perform sample derivatives--a few hours). It all depends on the method. I ran two peptide digestion methods that were similar to the LC/MS method (but could be considered "normal HPLC") for a protein of interest. We did protein digestions overnight, but the sample prep was very short. 5 minutes at the most. Run times for LC can be short (10 minutes is the shortest I've seen) to long (70 minutes). The peptide method I ran had a run time of 2.5 hrs which is also similar to the LC/MS method.

In general (or on average) LC/MS run times will be longer since you need to resolve many tiny little peaks.

Yes components are expensive for both. I believe typical LC system can be ~$50,000. I don't have a price for LCMS, but it was so pricey that our large company had to have a few months of backlogged work on the LCMS to justify the purchase of a second one (where as we had hundreds of HPLC systems

#3 DyDx

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Posted 23 March 2010 - 07:53 PM

All I can really add is that the mass spec is the expensive part. An HPLC system isn't so expensive.

#4 thanhlong

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Posted 16 November 2010 - 12:35 AM

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Edited by thanhlong, 16 November 2010 - 12:36 AM.


#5 thanhlong

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Posted 16 November 2010 - 12:37 AM

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