Hi all,
I have to measure proteasome activity by measuring fluorescence of degraded substrate Suc-LLVY-AMC. It is recommended to use 380-nm excitation and 460-nm emission filters. But the only available emission filters are 355-nm and 400-nm. Could anyone be so kind to advice on which is better to use, since I have failed to find an info on the subject? 7-Amino-4-methylcoumarin (AMCA) itself has 365-nm ex. Thanks in advance!
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#2
mdfenko
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Posted 29 January 2010 - 07:09 AM
use the 400nm filter. it will block light below 400nm and allow your wavelength through.
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genius does what it must
i do what i get paid to do
#3
katenkak
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Posted 29 January 2010 - 11:57 AM
Thanks!
mdfenko, on Jan 29 2010, 05:09 PM, said:
use the 400nm filter. it will block light below 400nm and allow your wavelength through.
#4
francesca
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Posted 24 August 2011 - 02:06 PM
Hi all,
I'm a new member of the Bioforum.
Some of you can suggest me how should be better to measure the proteasome activity in the brain tissue?
thanks in advance
I'm a new member of the Bioforum.
Some of you can suggest me how should be better to measure the proteasome activity in the brain tissue?
thanks in advance
#5
mdfenko
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Posted 24 August 2011 - 08:25 PM
do you have an assay for proteasome?
are you asking for a (partial) purification procedure?
are you asking for a (partial) purification procedure?
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
