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Fluorescent measurements-AMCA


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4 replies to this topic

#1 katenkak

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Posted 29 January 2010 - 06:32 AM

Hi all,
I have to measure proteasome activity by measuring fluorescence of degraded substrate Suc-LLVY-AMC. It is recommended to use 380-nm excitation and 460-nm emission filters. But the only available emission filters are 355-nm and 400-nm. Could anyone be so kind to advice on which is better to use, since I have failed to find an info on the subject? 7-Amino-4-methylcoumarin (AMCA) itself has 365-nm ex. Thanks in advance!

#2 mdfenko

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Posted 29 January 2010 - 07:09 AM

use the 400nm filter. it will block light below 400nm and allow your wavelength through.
talent does what it can
genius does what it must
i do what i get paid to do

#3 katenkak

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Posted 29 January 2010 - 11:57 AM

Thanks!

use the 400nm filter. it will block light below 400nm and allow your wavelength through.



#4 francesca

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Posted 24 August 2011 - 02:06 PM

Hi all,


I'm a new member of the Bioforum.

Some of you can suggest me how should be better to measure the proteasome activity in the brain tissue?

thanks in advance

#5 mdfenko

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Posted 24 August 2011 - 08:25 PM

do you have an assay for proteasome?

are you asking for a (partial) purification procedure?
talent does what it can
genius does what it must
i do what i get paid to do




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