SDS-PAGE after ammonium sulfate precipitation
Posted 28 January 2010 - 11:06 PM
Is it absolutely required to dialyse the dissolved ppt before loaading to a PAGE gel?
Will AS affect the electrophoresis so much? I just want to know if it is precipitated. I will not run the sup, just the ppt
Your responses will be very much appreciated
Posted 29 January 2010 - 03:27 AM
Posted 29 January 2010 - 07:15 AM
you could tca precipitate the protein after resuspending the pellet instead of dialyzing but that carries its own post-precipitation manipulations.
genius does what it must
i do what i get paid to do
Posted 31 January 2010 - 08:01 PM