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Nucleic acid markers for Nuclear Fractionation?


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#1 Baars01

Baars01

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Posted 28 January 2010 - 07:55 AM

Hi,

I am designing a nuclear fractionation experiment and will be looking at RNA in the fractions. To confirm the quality of the fractions I intend on using nucleic acid markers for negative and positive controls (as this is what I'll be studying).

As a positive and negative control for the nuclear and cytoplasmic fraction respectively, genomic DNA will probably be the ideal marker.

However, I have trouble finding a good negative control for the nuclear fraction and a good postive control for the cytoplasmic fraction.

I was thinking along the lines of ribosomal RNA, however this also exists in the nucleus. Perhaps focusing on RNA that has undergone extensive post-transcriptional modification could be another alternative.

Any ideas?




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