I express some flag and 6his tag exogenous genes in CHO cells and I need to quantify thier expression in different clones so I am going to coat my cell lysate on 96 well plates and do elise with anti tag antibody, but since tags are very small in comparision to whole cellular proteins I am not sure if it really works, I know I can use sandwich elisa in which antibodies against expressed proteins are coated on plates but it seems very expensive for my project.
is there any suggestion?
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setting an leisa for quantification of recombinant protein
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