Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

setting an leisa for quantification of recombinant protein

  • Please log in to reply
No replies to this topic

#1 sara.r



  • Active Members
  • PipPipPipPipPip
  • 95 posts

Posted 28 January 2010 - 06:45 AM

I express some flag and 6his tag exogenous genes in CHO cells and I need to quantify thier expression in different clones so I am going to coat my cell lysate on 96 well plates and do elise with anti tag antibody, but since tags are very small in comparision to whole cellular proteins I am not sure if it really works, I know I can use sandwich elisa in which antibodies against expressed proteins are coated on plates but it seems very expensive for my project.

is there any suggestion?

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.