I have a question regarding PCR and the nature of the template:
1) Good amplification if I use as template a previous amplified insert.
2) No product if the template is a miniprep product containing the insert cloned in a vector.
Why sometimes, not always, I am not able to get any PCR product using the second option.
Thanks in advance
Edited by celvas, 28 January 2010 - 06:46 AM.