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Ligated Vector Age

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#1 Epigenome



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Posted 27 January 2010 - 08:07 AM

Does anyone know if there is a broadly accepted "best if used by" date for a ligated vector? I have a set of pCR 2.1 ® ligations (that is, I have already completed my ligation reaction...insert is presumably......inserted) that I would like to revisit but am unsure if they are too old to be successful during transformation. They have been at -20 degrees for six months now....too old? Thanks everyone!

#2 swanny



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Posted 28 January 2010 - 05:51 PM

How long after the ligation reaction were the tubes frozen? Theoretically, there's no "best before" date if the samples were frozen, but there's only one way to find out... do the experiment.
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