Cell Lysis for DNA extraction
Posted 27 January 2010 - 12:06 AM
I've been processing my own batch of bacteria (Gram negative) without much problem and the DNA extraction were smooth.
Now, being given a batch of a few Gram positive bacteria, it's been frustrating getting them to break up. Of course, the Promega kit which I'm using suggested the addition of lysozyme. Problem now is what kind of lysozyme to use?
This link (Sigma) shows a great deal of lysozyme: http://www.sigmaaldr...p...;N25=0&F=PR
So, to those with experience breaking up the Gram positive bacteria, do help me out. Please? Other suggestions of lysis are welcomed.
Posted 27 January 2010 - 12:59 AM
Posted 27 January 2010 - 07:23 AM
genius does what it must
i do what i get paid to do
Posted 01 February 2010 - 04:48 AM