hey guys, i am trying to insert GFP that I isolated from a plasmid using NotI into the same plasmid but with an E22A mutation. All my controls worked but the transformation itself didn't work. For some reason i don't have growth even with a 10:1 insert on vector ratio. I use 100ul DH5a competent cells and 1ul of my ligation. I don't really know what's wrong, my controls tell me it should work. I even tried 3:1 and 5:1 to no success.
Anyone has any ideas?
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