Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

defatting of soybean powder for protein isolation


  • Please log in to reply
3 replies to this topic

#1 amoltej

amoltej

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 25 January 2010 - 12:40 AM

hi all ....
i have isolate proteinase inhibitors from the soybean.
can u plz suggest me the best method to remove the fats present5 in the soyaflour..
plz help me...

#2 Gerard

Gerard

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 124 posts
0
Neutral

Posted 25 January 2010 - 02:22 AM

For fat extraction from plants to extract proteins for allergenpreparation we use a soxlett extraction with ether followed bij frees drying.
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".

#3 amoltej

amoltej

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 25 January 2010 - 02:48 AM

For fat extraction from plants to extract proteins for allergenpreparation we use a soxlett extraction with ether followed bij frees drying.

hey hi!!!1
thanx but i hav query.....
in soxhlet we heat the solution which may adversly affect the proteins of my interest.....
and wht is allergenpreperation. wht is protocol for it? can u give me some more detail plz...
thanx for attention

#4 Gerard

Gerard

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 124 posts
0
Neutral

Posted 25 January 2010 - 06:03 AM

di-Ethylether has a boilingpoint of 35oC and will almost not harm proteins.
This extraction is done to remove the fats from the starting material i.e peanuts, the further protocol depends on the use for the starting material as elisa, blotting or skinprick to confirm a allergy for the starting material

Edited by Gerard, 25 January 2010 - 06:03 AM.

Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.