Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

how to remove coverslip for FISH after liquid N2 freezing


  • Please log in to reply
No replies to this topic

#1 peixumol

peixumol

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 34 posts
0
Neutral

Posted 25 January 2010 - 12:14 AM

Hi dear,
I am doing FISH these days. But I don't know how to remove coverslip after freezing the glass slide in liquid N2. I found it difficult to remove the coverslip when it was still frozen but when tried to remove it after thawing it always moved. can someone help? Thanks greatly.

Moreover, the protocol says to drop 200ul hybridization buffer onto the slide and to keep it at 42oC overnight. I wonder whether such a long time incubation would cause the buffer to dry out? Is it needed to film the slide or just cover with a coverslip being OK?

Many many many thanks!
paul in NanJing China
God helps them who help themselves!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.