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overnight?


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3 replies to this topic

#1 shanidr

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Posted 24 January 2010 - 11:34 AM

Hey all

I have a funny question, I hope its OK

When I put an andibody overnight in 4c , how long can I put it?
it will be ok if it will be for 24h?

thanks,
shani

#2 Gerard

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Posted 25 January 2010 - 02:43 AM

Most of my antibodys are stored at 4 degrees and others are frozen at -24 or -80 degrees for long times (years).
All depends on the antibody and the medium it dissolved in.
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".

#3 sgt4boston

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Posted 25 January 2010 - 03:50 AM

Overnight should be fine. For extended periods incorporate a preservative such as azide or thimerosal or proclin.

Some Mabs will precipitate if frozen.

#4 laurequillo

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Posted 26 January 2010 - 08:02 AM

Sometimes I just left the blot with the antibody at 4C for 48h (some weekends I forgot the blot!! :)) and it was ok (normally I add Azide to my primary antibody dilution)

Edited by laurequillo, 26 January 2010 - 08:03 AM.

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