Hey all
I have a funny question, I hope its OK
When I put an andibody overnight in 4c , how long can I put it?
it will be ok if it will be for 24h?
thanks,
shani
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3 replies to this topic
#2
Gerard
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Posted 25 January 2010 - 02:43 AM
Most of my antibodys are stored at 4 degrees and others are frozen at -24 or -80 degrees for long times (years).
All depends on the antibody and the medium it dissolved in.
All depends on the antibody and the medium it dissolved in.
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
#3
sgt4boston
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Posted 25 January 2010 - 03:50 AM
Overnight should be fine. For extended periods incorporate a preservative such as azide or thimerosal or proclin.
Some Mabs will precipitate if frozen.
Some Mabs will precipitate if frozen.
#4
laurequillo
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The Goddamn Batman!
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Posted 26 January 2010 - 08:02 AM
Sometimes I just left the blot with the antibody at 4ºC for 48h (some weekends I forgot the blot!! 
) and it was ok (normally I add Azide to my primary antibody dilution)
) and it was ok (normally I add Azide to my primary antibody dilution)
Edited by laurequillo, 26 January 2010 - 08:03 AM.
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