Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Quantifying histogram data


  • Please log in to reply
2 replies to this topic

#1 cardosopedro

cardosopedro

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 127 posts
3
Neutral

Posted 24 January 2010 - 08:06 AM

Hi.

What is the best method to quantify your data from flow cytometry histograms?

As far as I looked, you can use the mean fluorescence intensity to compare two experimental conditions. However, this is not always a good strategy. For instance, in my ROS measurements I get small (unespecific) peaks of fluorescence behind the main peak, and this affects the mean fluorescence.

Another possibility is to create and quantify regions at the right and left of a certain point. However, I never know how to decide where should be this reference point... :wacko:


Thanks for any reply.

#2 gradbiotech

gradbiotech

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 02 March 2010 - 06:49 PM

Hi there,

I am willing to compare GFP amounts in different samples analyzed through FACS:

sample 1: histogram analysis

Marker Left, Right Events % Gated % Total Mean Geo Mean CV Median Peak Ch
All 1, 9910 5000 100.00 100.00 177.79 8.01 639.27 7.17 1
M1 11, 9910 1500 33.33 33.33 525.62 33.24 344.98 16.70 9910

sample 2: histogram analysis

All 1, 9910 5000 100.00 100.00 1620.37 51.29 203.12 16.85 9910
M1 11, 9910 2570 50.21 50.00 2813.57 343.81 139.85 230.82 9910

Is there anyway to plot RFU plot between them. It is evident that sample 2 has far more cells expressing GFP than sample 1

#3 gradbiotech

gradbiotech

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 02 March 2010 - 07:01 PM

sorry table messed up:

please find attached file for data "for forum"

Attached Files






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.